Search results for "Vitellogenin 1"

showing 2 items of 2 documents

Use of Early Life-Stages of Zebrafish to Assess Toxicity of Sediments Contaminated by Organotin Compounds

2016

ABSTRACTThis study examined the response of early life-stages (ELS) of zebrafish to organotin-contaminated sediment from Lake Huruslahti (HL) in Central Finland. A dilution series (0, 10, 33, and 100%) of the native (HL) and the sediment spiked with tributyltin (TBT) determined a dose-response of zebrafish ELS to organotin-contaminated sediment. Sediment elutriates were assessed by bacterial bioluminescence assay and microscopical pathologies of 1–3 days post-fertilization zebrafish (1–3dpfZF). Brain aromatase (cyp19a1b) and tissue vitellogenin (vtg1) were assayed from early-juvenile zebrafish (20dpfZF) exposed to intact sediment. In vivo modulation of cyp19a1b and vtg1 transcripts in 20dpf…

0301 basic medicineneural aromatase (cyp19a1b)Health Toxicology and Mutagenesista1172Soil Science010501 environmental sciences01 natural sciencessediment assay in vivotributyltin03 medical and health scienceschemistry.chemical_compoundVitellogeninIn vivoEnvironmental ChemistryBioluminescenceZebrafish0105 earth and related environmental sciencesbiologySedimentAquatic animalbiology.organism_classificationzebrafishPollutionMolecular biologyvitellogenin 1 (vtg1)030104 developmental biologychemistryEnvironmental chemistryToxicityTributyltinbiology.proteinSoil and Sediment Contamination
researchProduct

Use of Early Juvenile Zebrafish Danio Rerio for In-Vivo Assessment of Endocrine Modulation by Xenoestrogens

2013

Reliable and cost-effective early-life stage (ELS) bioassays incorporating practical experimentation without compromising scientific relevance are crucial in chemical risk assessment. This study investigated the use of 20 days- post-fertilization life stage ( 20dpfZF ) of zebrafish Danio rerio to screen environmental chemicals known to be estrogenic in adult fish. Firstly, studies with key genes in steroidogenesis were conducted; the brain isoform of aromatase gene ( cyp19a1b ) being the most prominently expressed biomarker. Regulation of mRNA levels of molecular biomarkers, vitellogenin 1 gene ( vtg1) and cyp19a1b were selected to assess the endocrine modulation by xenoestrogens, 17α-ethin…

brain aromataseVitellogenin 1Xenoestrogenicityearly life stageZebrafish
researchProduct